DNA

Part:BBa_K1614023

Designed by: Daniel Heid   Group: iGEM15_Heidelberg   (2015-09-18)

Actin AptaBody with software generated actin aptamer (8mer)

MAWS software generated actin aptamer (8mer) linked with a 5x A linker to the horseradish peroxidase mimic DNAzyme.

This part comprises an aptamer designed to targed actin using MAWS a linker of AAAAA and the HRP-DNAzyme. It is capable of providing luminescence readout in the presence of hydrogen peroxide, and designed for Western blotting. Degradation of hydrogen peroxide needed for luminescent readout with luminol requires the presence of hemin, with optimal behaviour at equimolar concentration of hemin and the construct. The optimal pH for the HRP-DNAzyme used in this construct is pH 8.5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None